KIF5C(1-560)-2xmCitrine-GFP11
(Plasmid
#61667)
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PurposeTandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61667 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneC1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4013
- Total vector size (bp) 6810
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KIF5C(1-560)-2xmCitrine-GFP11 was a gift from Kristen Verhey (Addgene plasmid # 61667 ; http://n2t.net/addgene:61667 ; RRID:Addgene_61667) -
For your References section:
A method for multiprotein assembly in cells reveals independent action of kinesins in complex. Norris SR, Soppina V, Dizaji AS, Schimert KI, Sept D, Cai D, Sivaramakrishnan S, Verhey KJ. J Cell Biol. 2014 Nov 10;207(3):393-406. doi: 10.1083/jcb.201407086. Epub 2014 Nov 3. 10.1083/jcb.201407086 PubMed 25365993