SNAP-EF(N)-5nmSAH-GFP(1-10)
(Plasmid
#61659)
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Purpose5nm SAH scaffold with EF Hand and split GFP attachment sites
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61659 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneC1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4013
- Total vector size (bp) 5577
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSNAP-EF(N)-5nmSAH-GFP(1-10)
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Insert Size (bp)1641
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFP(1-10) sequence provided by F Pinaud [(2011) PNAS 108:E301-E210] based on work in the lab of G Waldo engineering the split GFP to function as a self assembling bipartite protein [Cabantous et al. (2005) Nat Biotechnol 23:102–107].
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SNAP-EF(N)-5nmSAH-GFP(1-10) was a gift from Kristen Verhey (Addgene plasmid # 61659 ; http://n2t.net/addgene:61659 ; RRID:Addgene_61659) -
For your References section:
A method for multiprotein assembly in cells reveals independent action of kinesins in complex. Norris SR, Soppina V, Dizaji AS, Schimert KI, Sept D, Cai D, Sivaramakrishnan S, Verhey KJ. J Cell Biol. 2014 Nov 10;207(3):393-406. doi: 10.1083/jcb.201407086. Epub 2014 Nov 3. 10.1083/jcb.201407086 PubMed 25365993
Map uploaded by the depositor.
