pFPL-Rg
(Plasmid #61654)

• Purpose: Gateway vector for generating red fluorescent protein fusions in fungi
• Depositing Lab: Mark Farman
• Publication: Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Backbone

• Vector backbone: pCAMBIA1300
• Backbone manufacturer: Cambia, Canberra, Australia
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 11500
• Modifications to backbone: Additions to T-DNA region: insertion of selectable marker (geneticin resistance), gateway cassette, GFP gene, fungal terminator sequences.
• Vector type: Plant Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: monomeric red fluorescent protein
• Alt name: mRFP
• Species: Discosoma sp.
• Insert Size (bp): 678

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry