pFPNL1
(Plasmid
#61651)
-
PurposeGateway vector for generating nucleus-targeted fluorescent protein fusions in fungi
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 61651 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCAMBIA1300
-
Backbone manufacturerCambia, Canberra, Australia
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 11500
-
Modifications to backboneAdditions to T-DNA region: insertion of selectable marker (hygromycin resistance), gateway cassette, GFP gene, fungal terminator sequences.
-
Vector typePlant Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namemonomeric red fluorescent protein
-
Alt namemRFP
-
SpeciesDiscosoma sp.
-
Insert Size (bp)678
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFPNL1 was a gift from Mark Farman (Addgene plasmid # 61651 ; http://n2t.net/addgene:61651 ; RRID:Addgene_61651) -
For your References section:
pFPL vectors for high-throughput protein localization in fungi: detecting cytoplasmic accumulation of putative effector proteins. Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin MM, Farman M. Mol Plant Microbe Interact. 2014 Nov 12. 10.1094/MPMI-05-14-0144-TA PubMed 25390188