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Addgene

pFPL-Ch
(Plasmid #61647)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61647 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAMBIA1300
  • Backbone manufacturer
    Cambia, Canberra, Australia
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 11500
  • Modifications to backbone
    Additions to T-DNA region: insertion of selectable marker (hygromycin resistance), gateway cassette, GFP gene, fungal terminator sequences.
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    enhanced cyan fluorescent protein
  • Alt name
    eCFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    720
  • GenBank ID

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFPL-Ch was a gift from Mark Farman (Addgene plasmid # 61647 ; http://n2t.net/addgene:61647 ; RRID:Addgene_61647)

  • For your References section:

    pFPL vectors for high-throughput protein localization in fungi: detecting cytoplasmic accumulation of putative effector proteins. Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin MM, Farman M. Mol Plant Microbe Interact. 2014 Nov 12. 10.1094/MPMI-05-14-0144-TA PubMed 25390188
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