pCDNAIIIB rac1 QL
(Plasmid #61633)

• Purpose: mammalian expression of rac1 constitutively active mutant
• Depositing Lab: JS Gutkind
• Publication: Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Backbone

• Vector backbone: pcDNA3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rac1
• Alt name: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
• Species: H. sapiens (human)
• Insert Size (bp): 600
• Mutation: Q61L, constitutively active
• Entrez Gene: RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry