pCDNAIIIB rhoA QL
(Plasmid #61632)

• Purpose: mammalian expression of rhoA constitutively active mutant
• Depositing Lab: JS Gutkind
• Publication: Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Backbone

• Vector backbone: pcDNA3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rhoA
• Alt name: ras homolog family member A
• Species: H. sapiens (human)
• Insert Size (bp): 600
• Mutation: Q63L, constitutively active
• Entrez Gene: RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry