pcDNA3-AKAR4-Kras
(Plasmid #61621)

• Purpose: Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity
• Depositing Lab: Jin Zhang
• Publication: Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10.1039/c0mb00079e. Epub 2010 Sep 14.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Total vector size (bp): 7484
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AKAR4-Kras
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 1980
• Promoter: CMV
• Tags / Fusion Proteins:
  • Cerulean (N terminal on insert)
  • cpVenus[E172] (C terminal on insert)
  • C-terminal targeting sequence from Kras (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-AKAR4-Kras
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry