DPYD-FLAG
(Plasmid #61616)

• Purpose: The human DPYD was used to study the effect of DPYD expression on EMT
• Depositing Lab: David Sabatini
• Publication: Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi: 10.1016/j.cell.2014.07.032.

Backbone

• Vector backbone: pLJC2
• Backbone size w/o insert (bp): 7347
• Total vector size (bp): 10512
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dihydropyrimidine dehydrogenase
• Alt name: DPYD
• Species: H. sapiens (human)
• Insert Size (bp): 3165
• GenBank ID: NM_000110.3 NM_001160301.1
• Entrez Gene: DPYD (a.k.a. DHP, DHPDHASE, DPD, DYPD)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: TGTACGGTGGGAGGTCTATATAAG
• 3′ sequencing primer: CCCTTTTCTTTTAAAATTGTGGATGAATACTGCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ORFeome

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry