pLJM5-I560S-mDPYD
(Plasmid #61615)

• Purpose: The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT
• Depositing Lab: David Sabatini
• Publication: Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi: 10.1016/j.cell.2014.07.032.

Backbone

• Vector backbone: pLJM5
• Backbone size w/o insert (bp): 8001
• Total vector size (bp): 11079
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dihydropyrimidine dehydrogenase
• Alt name: Dpyd
• Species: M. musculus (mouse)
• Insert Size (bp): 3086
• Mutation: Changed Isoleucine at position 560 to Serine
• Entrez Gene: Dpyd (a.k.a. AI315208, DPD, E330028L06Rik)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: TGTACGGTGGGAGGTCTATATAAG
• 3′ sequencing primer: CCCTTTTCTTTTAAAATTGTGGATGAATACTGCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Thermo Scientific Biochem Sciences (Catalog Number: 4235971)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry