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Addgene

pET28a-LSSmOrange-CNA35
(Plasmid #61605)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61605 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21(DE3)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LSSmOrange and CNA35
  • Promoter T7
  • Tag / Fusion Protein
    • His tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CNA35 was a kind gift from Dr Magnus Hook (Texas A&M)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-LSSmOrange-CNA35 was a gift from Maarten Merkx (Addgene plasmid # 61605 ; http://n2t.net/addgene:61605 ; RRID:Addgene_61605)

  • For your References section:

    Colorful Protein-Based Fluorescent Probes for Collagen Imaging. Aper SJ, van Spreeuwel AC, van Turnhout MC, van der Linden AJ, Pieters PA, van der Zon NL, de la Rambelje SL, Bouten CV, Merkx M. PLoS One. 2014 Dec 9;9(12):e114983. doi: 10.1371/journal.pone.0114983. eCollection 2014. PONE-D-14-29186 [pii] PubMed 25490719
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