pET28a-EGFP-CNA35
(Plasmid #61603)

• Purpose: bacterial expression of N-terminal fusion protein of CNA35 with EGFP
• Depositing Lab: Maarten Merkx
• Publication: Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi: 10.1371/journal.pone.0114983. eCollection 2014.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BL21(DE3)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP and CNA35
• Promoter: T7
• Tag / Fusion Protein:
  • His tags

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (unknown if destroyed)
• 3′ cloning site: Xho1 (unknown if destroyed)
• 5′ sequencing primer: T7 promotor

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CNA35 gene was a kind gift of Prof Magnus Hook (Texas A&M)
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry