pEPkan-S2
(Plasmid #61601)

• Purpose: PCR template for en passant (two-step Red-mediated) mutagenesis
• Depositing Lab: Nikolaus Osterrieder
• Publication: Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Backbone

• Vector backbone: pcDNA3
• Total vector size (bp): 6441

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kana_I-SceI

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SP6

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry