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Addgene

pMPRAlenti1
(Plasmid #61600)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61600 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 8658
  • Modifications to backbone
    Insertion of cat-ccdB cassette flanked by two distinct SfiI restriction sites for directional cloning of pMPRA libraries.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    cat-ccdB cassette also confers chloramphenicol resistance in bacteria
  • Copy number
    High Copy

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cat-ccdB insert was PCR cloned from pLenti obtained from Addgene
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMPRAlenti1 was a gift from Tarjei Mikkelsen (Addgene plasmid # 61600 ; http://n2t.net/addgene:61600 ; RRID:Addgene_61600)
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