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pOPINK-TRABID (OTU+AnkUBD, aa 245-697)
(Plasmid #61590)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61590 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOPINK
  • Backbone manufacturer
    OPPF-UK
  • Backbone size w/o insert (bp) 6299
  • Total vector size (bp) 7329
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TRABID
  • Alt name
    Ubiquitin thioesterase ZRANB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1359
  • Mutation
    Deleted aa 1-244 and aa 698-708.
  • GenBank ID
    NM_017580.2
  • Entrez Gene
    ZRANB1 (a.k.a. TRABID)
  • Promoter T7
  • Tag / Fusion Protein
    • His6-GST-3C (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pGEX 5’
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.

Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINK-TRABID (OTU+AnkUBD, aa 245-697) was a gift from David Komander (Addgene plasmid # 61590 ; http://n2t.net/addgene:61590 ; RRID:Addgene_61590)

  • For your References section:

    An ankyrin-repeat ubiquitin-binding domain determines TRABID's specificity for atypical ubiquitin chains. Licchesi JD, Mieszczanek J, Mevissen TE, Rutherford TJ, Akutsu M, Virdee S, El Oualid F, Chin JW, Ovaa H, Bienz M, Komander D. Nat Struct Mol Biol. 2011 Dec 11;19(1):62-71. doi: 10.1038/nsmb.2169. 10.1038/nsmb.2169 PubMed 22157957
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