Skip to main content
Addgene

pOPINK-vOTU (CCHFV OTU, aa 1-183)
(Plasmid #61589)

Print

Ordering

This material is available to academics and nonprofits only. Availability may be limited outside the U.S. Please log in for more information.
Item Catalog # Description Quantity Price (USD)
Plasmid 61589 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pOPINK
  • Backbone manufacturer
    OPPF-UK
  • Backbone size w/o insert (bp) 6299
  • Total vector size (bp) 6519
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    vOTU (viral OTU)
  • Alt name
    Protein L
  • Alt name
    RNA-directed RNA polymerase L
  • Species
    Crimean-Congo hemorrhagic fever virus (CCHFV)
  • Insert Size (bp)
    549
  • Promoter T7
  • Tag / Fusion Protein
    • His6-GST-3C (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (destroyed during cloning)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer pGEX 5’
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.

Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINK-vOTU (CCHFV OTU, aa 1-183) was a gift from David Komander (Addgene plasmid # 61589)

  • For your References section:

    Molecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domains. Akutsu M, Ye Y, Virdee S, Chin JW, Komander D. Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2228-33. doi: 10.1073/pnas.1015287108. Epub 2011 Jan 25. 10.1073/pnas.1015287108 PubMed 21266548
Related items: