pGEX6P1-A20 (OTU, aa 1-366)
(Plasmid #61584)

• Purpose: Expresses human A20 (OTU domain) in E. coli.
• Depositing Lab: David Komander
• Publication: Komander et al Biochem J. 2008 Jan 1;409(1):77-85.

Backbone

• Vector backbone: pGEX6P1
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4984
• Total vector size (bp): 6062
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: A20
• Alt name: TNF alpha-induced protein 3
• Species: H. sapiens (human)
• Insert Size (bp): 1098
• Mutation: Deleted aa 367-790.
• GenBank ID: NM_001270508.1
• Entrez Gene: TNFAIP3 (a.k.a. A20, AIFBL1, AISBL, OTUD7C, TNFA1P2)
• Promoter: tac
• Tag / Fusion Protein:
  • GST-3C (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pGEX 5’
• 3′ sequencing primer: pGEX 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.