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Purpose(Empty Backbone) Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAMD1-4
- Backbone size (bp) 4730
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Vector typeSynthetic Biology ; Mini-Tn5 vector
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Selectable markersAmpicillin
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAMD1-4 was a gift from Víctor de Lorenzo (Addgene plasmid # 61565 ; http://n2t.net/addgene:61565 ; RRID:Addgene_61565) -
For your References section:
New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Martinez-Garcia E, Aparicio T, de Lorenzo V, Nikel PI. Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014. 10.3389/fbioe.2014.00046 PubMed 25389526
Map uploaded by the depositor.
