pBAMD1-2
(Plasmid #61564)

• Purpose: (Empty Backbone) Standardized mini-Tn5 delivery plasmid, kanamycin resistant
• Depositing Lab: Víctor de Lorenzo
• Publication: Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014.

Backbone

• Vector backbone: pBAMD1-2
• Backbone size (bp): 4658
• Vector type: Synthetic Biology ; Mini-Tn5 vector
• Selectable markers: Ampicillin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: Low Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.