pBAD-pHoran4
(Plasmid
#61558)
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PurposeOrange fluorescent protein based pH indicator in bacterial expression vector
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61558 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD/His-B
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Backbone manufacturerInvitrogen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepHoran4
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Alt namemOrange M163K
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SpeciesSynthetic
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Insert Size (bp)700
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBAD-Fwd
- 3′ sequencing primer pBAD-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-pHoran4 was a gift from Robert Campbell (Addgene plasmid # 61558 ; http://n2t.net/addgene:61558 ; RRID:Addgene_61558) -
For your References section:
pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis. Shen Y, Rosendale M, Campbell RE, Perrais D. J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107. 10.1083/jcb.201404107 PubMed 25385186