pRSETb-pHlash
(Plasmid #61550)

• Purpose: Fusion of Rluc8 and cpVenus. Expression of pHlash in Escherichia coli for protein purification
• Depositing Lab: Carl Johnson
• Publication: Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371/journal.pone.0043072. Epub 2012 Aug 14.

Backbone

• Vector backbone: pRSETb
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 4580
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pHlash
• Alt name: Rluc8-cpVenus
• Species: Synthetic
• Insert Size (bp): 1680
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: AGGATCCAATGGCTTCCAAGGTGTACGA
• 3′ sequencing primer: CGCGAATTCTTACTCGATGTTGTGGCGGA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Fusion components were requested from collaborator sources. The complete vector was constructed by the submitting party.