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PurposeThe 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61549 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM3Z
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name1.3 times of the HBV genome with luciferase within the Core ORF
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Insert Size (bp)3932
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MutationSubstitution of HBV sequences with luciferase ORF. No vector mammalian promoters. HBV inherent enhancer and promoters
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositing laboratory has indicated that this plasmid does not produce virus since it lacks the polymerase/RTase gene. It is recommend to consult with your institutions Biosafety Committee prior to use, as different institutions may have specific requirements for usage.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
1.3xWT HBV-Luciferase was a gift from Yosef Shaul (Addgene plasmid # 61549 ; http://n2t.net/addgene:61549 ; RRID:Addgene_61549) -
For your References section:
Enhancer I predominance in hepatitis B virus gene expression. Doitsh G, Shaul Y. Mol Cell Biol. 2004 Feb;24(4):1799-808. PubMed 14749394
Map uploaded by the depositor.
