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PurposeHuman transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61547 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTransferrin receptor
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SpeciesH. sapiens (human)
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Entrez GeneTFRC (a.k.a. CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90)
- Promoter CMV
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Tag
/ Fusion Protein
- pHoran4 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TfR-pHoran4 was a gift from David Perrais (Addgene plasmid # 61547 ; http://n2t.net/addgene:61547 ; RRID:Addgene_61547) -
For your References section:
pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis. Shen Y, Rosendale M, Campbell RE, Perrais D. J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107. 10.1083/jcb.201404107 PubMed 25385186
