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PurposeExpression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61524 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneC1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namelysosomal-associated membrane protein 1
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SpeciesH. sapiens (human)
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Entrez GeneLAMP1 (a.k.a. CD107a, LAMPA, LGP120)
- Promoter CMV
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Tags
/ Fusion Proteins
- mCherry (C terminal on insert)
- AIP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer SV40 poly A Reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LAMP-mChF-AIP was a gift from Takanari Inoue (Addgene plasmid # 61524 ; http://n2t.net/addgene:61524 ; RRID:Addgene_61524) -
For your References section:
Compartmentalized AMPK signaling illuminated by genetically encoded molecular sensors and actuators. Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, Chen M, Tokumitsu H, Zhang J, Inoue T. Cell Rep. 2015 Apr 28;11(4):657-70. doi: 10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16. 10.1016/j.celrep.2015.03.057 PubMed 25892241