Tom20-mChF-AIP(TE)
(Plasmid #61522)

• Purpose: Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutation
• Depositing Lab: Takanari Inoue
• Publication: Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi: 10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.

Backbone

• Vector backbone: C1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FK506 binding protein 1A
• Species: H. sapiens (human)
• Entrez Gene: FKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mCherry (N terminal on insert)
  • Tom20 (N terminal on insert)
  • AIP with TE mutation (see comments) (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: SV40 poly A Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

T429E in AIP following (in-frame with) FKBP caused by GA mismatch at 1313 bp, AC mismatch at 1314 bp, and AT mismatch at 1315 bp in Author's partial sequence (please refer to Tom20-mChF-AIP for comparison).