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Addgene

pERB221
(Plasmid #61502)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61502 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEM791
  • Backbone manufacturer
    Dr. Eugene Makeyev
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CenpB-GFP-Halo
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    mCherry-DHFR
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pERB221 was a gift from David Chenoweth & Michael Lampson (Addgene plasmid # 61502 ; http://n2t.net/addgene:61502 ; RRID:Addgene_61502)

  • For your References section:

    Localized light-induced protein dimerization in living cells using a photocaged dimerizer. Ballister ER, Aonbangkhen C, Mayo AM, Lampson MA, Chenoweth DM. Nat Commun. 2014 Nov 17;5:5475. doi: 10.1038/ncomms6475. 10.1038/ncomms6475 PubMed 25400104
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