pERB215
(Plasmid
#61499)
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PurposeConst. Halotag-GFP-Nuf2 + mCherry-DHFR
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 61499 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEM791
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Backbone manufacturerDr. Eugene Makeyev
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameHaloTag-GFP-Nuf2
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- HaloTag (N terminal on insert)
Gene/Insert 2
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Gene/Insert namemCherry-DHFR
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pERB215 was a gift from David Chenoweth & Michael Lampson (Addgene plasmid # 61499 ; http://n2t.net/addgene:61499 ; RRID:Addgene_61499) -
For your References section:
Localized light-induced protein dimerization in living cells using a photocaged dimerizer. Ballister ER, Aonbangkhen C, Mayo AM, Lampson MA, Chenoweth DM. Nat Commun. 2014 Nov 17;5:5475. doi: 10.1038/ncomms6475. 10.1038/ncomms6475 PubMed 25400104