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Addgene

pAD-g6
(Plasmid #61497)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61497 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR50
  • Backbone manufacturer
    a gift of C. Richie and A. Golden, National Institutes of Health
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gld-1
  • Alt name
    Cbr-gld-1
  • Species
    Caenorhabditis briggsae
  • Entrez Gene
    Cbr-gld-1 (a.k.a. CBG00303)
  • Promoter gld1
  • Tags / Fusion Proteins
    • His (C terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert contains 923 nt upstream of start codon and 1,354 nt downstream of stop codon.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAD-g6 was a gift from Eric Haag (Addgene plasmid # 61497 ; http://n2t.net/addgene:61497 ; RRID:Addgene_61497)

  • For your References section:

    Independent recruitments of a translational regulator in the evolution of self-fertile nematodes. Beadell AV, Liu Q, Johnson DM, Haag ES. Proc Natl Acad Sci U S A. 2011 Dec 6;108(49):19672-7. doi: 10.1073/pnas.1108068108. Epub 2011 Nov 21. 10.1073/pnas.1108068108 PubMed 22106259
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