RAB14 3'UTR D2
(Plasmid #61491)

• Purpose: luciferase reporter containing RAB14 3' UTR with the second predicted MiR144 binding site deleted
• Depositing Lab: Curt Civin
• Publication: Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164.

Backbone

• Vector backbone: pmirGLO Dual-Luciferase
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 7350
• Total vector size (bp): 8150
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RAB14 3' UTR
• Alt name: RAB14
• Species: H. sapiens (human)
• Insert Size (bp): 801
• Mutation: contains position 837-1637 (numbering relative to start of 3'utr) and second predicted MIR144 binding site deleted, 10bp deletion
• GenBank ID: NM_016322
• Entrez Gene: RAB14 (a.k.a. FBP, RAB-14)
• Promoter: PGK
• Tag / Fusion Protein:
  • luciferase (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: Luc-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Oligos used for site-directed mutagenesis:
F-CAGGTGGTTTGGCCACTGGCAAGAGAAACAATCTTA
R-TAAGATTGTTTCTCTTGCCAGTGGCCAAACCACCTG

These 10bp deletion constructs were used in BJH (2015).