F11-R pEBio
(Plasmid #61486)

• Purpose: Expresses full-length F11R (JAM1) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag and biotinylation sequence
• Depositing Lab: Gavin Wright
• Publication: Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.

Backbone

• Vector backbone: pEBio
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: F11R
• Alt name: JAMA
• Alt name: JAM1
• Species: H. sapiens (human)
• Insert Size (bp): 688
• Entrez Gene: F11R (a.k.a. CD321, JAM, JAM1, JAMA, JCAM, KAT, PAM-1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • rat CD4 d3+4 (C terminal on backbone)
  • enzymatic biotinylation sequence (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert was chemically synthesised