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Addgene

pEn-C1.1
(Plasmid #61479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-T-easy
  • Backbone manufacturer
    Promega Corp.
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 3700
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtU6-26:sgRNA
  • Species
    A. thaliana (mustard weed), Synthetic
  • Promoter U6-26

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Based on pEn-Chimera; MluI and Bsu36I RS added to each side of the U6-26:sgRNA sequence for cloning of up to 3 sgRNAs into Cas9 expression vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEn-C1.1 was a gift from Holger Puchta (Addgene plasmid # 61479 ; http://n2t.net/addgene:61479 ; RRID:Addgene_61479)

  • For your References section:

    The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny. Schiml S, Fauser F, Puchta H. Plant J. 2014 Oct 18. doi: 10.1111/tpj.12704. 10.1111/tpj.12704 PubMed 25327456
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