pDe-CAS9-D10A
(Plasmid
#61477)
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Purposebased on pDe-CAS9, encodes Cas9 D10A nickase variant
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPZP221
- Backbone size w/o insert (bp) 7100
- Total vector size (bp) 15800
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Vector typePlant Expression, CRISPR
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Growth instructionscontains ccdB gene
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9-D10A
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)4100
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Mutationcatalytic Asp10 changed to Ala
- Promoter PcUbi4-2
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 rev
- 3′ sequencing primer M13 fw (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDe-CAS9-D10A was a gift from Holger Puchta (Addgene plasmid # 61477 ; http://n2t.net/addgene:61477 ; RRID:Addgene_61477) -
For your References section:
Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Fauser F, Schiml S, Puchta H. Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17. 10.1111/tpj.12554 PubMed 24836556
Map uploaded by the depositor.
