pAAV-CW3SL-EGFP
(Plasmid #61463)

• Purpose: AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)
• Depositing Lab: Bong-Kiun Kaang
• Publication: Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.1186/1756-6606-7-17.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 3756
• Total vector size (bp): 4476
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFP
• Insert Size (bp): 720
• Promoter: CaMKIIa

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Resource Information

• Supplemental Documents:
  • pAAV-CW3SL-GFP.gb
  • Annotated Sequence
• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Feature annotations:

WPRE3 (1303 ~ 1553) -
gATAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTAGTTCTTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTT

SV40 late Poly(A) signal (1554 ~ 1734) -
TATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATCTAGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGCGG