pEX-PK-hLC3
(Plasmid #61458)

• Purpose: Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy
• Depositing Lab: Isei Tanida
• Publication: Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi: 10.1371/journal.pone.0110600. eCollection 2014.

Backbone

• Vector backbone: pCXN2/ pCAGGS
• Backbone size w/o insert (bp): 7000
• Total vector size (bp): 9000
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PK-hLC3
• Alt name: pHluorin-mKate2-hLC3
• Species: H. sapiens (human), S. pombe (fission yeast)
• Entrez Gene: MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: GGCTTCTGGCGTGTGACC
• 3′ sequencing primer: AGCCACCACCTTCTGATAG

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We thank Dr. James Edward Rothman for providing respective pHluorin.