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Addgene

pDe-CAS9
(Plasmid #61433)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61433 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPZP221
  • Backbone size w/o insert (bp) 7100
  • Total vector size (bp) 15800
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    contains ccdB gene
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    4100
  • Promoter PcUBI4-2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer M13rev
  • 3′ sequencing primer M13fw
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDe-CAS9 was a gift from Holger Puchta (Addgene plasmid # 61433 ; http://n2t.net/addgene:61433 ; RRID:Addgene_61433)

  • For your References section:

    Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Fauser F, Schiml S, Puchta H. Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17. 10.1111/tpj.12554 PubMed 24836556
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