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Addgene

pEn-Chimera
(Plasmid #61432)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61432 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-T-easy
  • Backbone manufacturer
    Promega Corp.
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 3700
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtU6-26:sgRNA
  • gRNA/shRNA sequence
    to be specified by cloning
  • Species
    A. thaliana (mustard weed), Synthetic
  • Promoter AtU6-26

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEn-Chimera was a gift from Holger Puchta (Addgene plasmid # 61432 ; http://n2t.net/addgene:61432 ; RRID:Addgene_61432)

  • For your References section:

    Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Fauser F, Schiml S, Puchta H. Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17. 10.1111/tpj.12554 PubMed 24836556
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