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Addgene

Flag-mPer2ER-pBABEpuro
(Plasmid #61430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61430 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mPER2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4764
  • Entrez Gene
    Per2 (a.k.a. mKIAA0347, mPer2)
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • mouse estrogen receptor ligand binding domain (G525R) on C-term (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBabe 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-mPer2ER-pBABEpuro was a gift from Aziz Sancar (Addgene plasmid # 61430 ; http://n2t.net/addgene:61430 ; RRID:Addgene_61430)

  • For your References section:

    Dual modes of CLOCK:BMAL1 inhibition mediated by Cryptochrome and Period proteins in the mammalian circadian clock. Ye R, Selby CP, Chiou YY, Ozkan-Dagliyan I, Gaddameedhi S, Sancar A. Genes Dev. 2014 Sep 15;28(18):1989-98. doi: 10.1101/gad.249417.114. 10.1101/gad.249417.114 PubMed 25228643
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