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pOPINK-OTUB1 (full-length, aa 1-271)
(Plasmid #61420)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61420 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOPINK
  • Backbone manufacturer
    OPPF-UK
  • Backbone size w/o insert (bp) 6299
  • Total vector size (bp) 6783
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OTUB1 (Ubiquitin thioesterase OTUB1)
  • Alt name
    Otubain-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    813
  • GenBank ID
    NM_017670.2
  • Entrez Gene
    OTUB1 (a.k.a. HSPC263, OTB1, OTU1)
  • Promoter T7
  • Tag / Fusion Protein
    • His6-GST-3C (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pGEX 5’
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINK-OTUB1 (full-length, aa 1-271) was a gift from David Komander (Addgene plasmid # 61420 ; http://n2t.net/addgene:61420 ; RRID:Addgene_61420)

  • For your References section:

    OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and Enable Ubiquitin Chain Restriction Analysis. Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, Ovaa H, Komander D. Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046. 10.1016/j.cell.2013.05.046 PubMed 23827681
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