pOPINK-OTUD3 (OTU+UBA, aa 52-275)
(Plasmid #61411)

• Purpose: Expresses human OTUD3 (OTU+UBA domains) in E. coli.
• Depositing Lab: David Komander
• Publication: Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.

Backbone

• Vector backbone: pOPINK
• Backbone manufacturer: OPPF-UK
• Backbone size w/o insert (bp): 6299
• Total vector size (bp): 6642
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: OTUD3
• Species: H. sapiens (human)
• Insert Size (bp): 672
• Mutation: Deleted aa 1-51 and aa 276-398.
• GenBank ID: NM_015207.1
• Entrez Gene: OTUD3 (a.k.a. DUBA4)
• Promoter: T7
• Tag / Fusion Protein:
  • His6-GST-3C (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (destroyed during cloning)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pGEX 5’
• 3′ sequencing primer: T7 Terminal

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry