pOPINK-OTUD2 (OTU, aa 132-314)
(Plasmid
#61410)
-
PurposeExpresses human OTUD2 (OTU domain) in E. coli.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 61410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepOPINK
-
Backbone manufacturerOPPF-UK
- Backbone size w/o insert (bp) 6299
- Total vector size (bp) 6519
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameOTUD2
-
SpeciesH. sapiens (human)
-
Insert Size (bp)549
-
MutationDeleted aa 1-131 and aa 315-348.
-
GenBank IDNM_018566.3
-
Entrez GeneYOD1 (a.k.a. DUBA8, OTUD2, PRO0907)
- Promoter T7
-
Tag
/ Fusion Protein
- His6-GST-3C (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (destroyed during cloning)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pGEX 5’
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pOPINK-OTUD2 (OTU, aa 132-314) was a gift from David Komander (Addgene plasmid # 61410 ; http://n2t.net/addgene:61410 ; RRID:Addgene_61410) -
For your References section:
OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and Enable Ubiquitin Chain Restriction Analysis. Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, Ovaa H, Komander D. Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046. 10.1016/j.cell.2013.05.046 PubMed 23827681