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pmCherry_C1 I3(resist)
(Plasmid #61399)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP_C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5100
  • Modifications to backbone
    GFP was exchanged by mCherry via restriction digest with AgeI and EcoRI
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Inhibitor-3
  • Alt name
    PPP1R11
  • Species
    H. sapiens (human)
  • Mutation
    silent mutations of bases C183T T192C T189C
  • Entrez Gene
    PPP1R11 (a.k.a. CFAP255, HCG-V, HCGV, IPP3, TCTE5, TCTEX5)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pEGFP-RP
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

resistant against siRNA oligomer I3 S2 (CUGCUGTAUUUAUGAGAAA)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry_C1 I3(resist) was a gift from Hemmo Meyer (Addgene plasmid # 61399 ; http://n2t.net/addgene:61399 ; RRID:Addgene_61399)

  • For your References section:

    Inhibitor-3 ensures bipolar mitotic spindle attachment by limiting association of SDS22 with kinetochore-bound protein phosphatase-1. Eiteneuer A, Seiler J, Weith M, Beullens M, Lesage B, Krenn V, Musacchio A, Bollen M, Meyer H. EMBO J. 2014 Oct 8. pii: e201489054. 10.15252/embj.201489054 PubMed 25298395
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