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pCS2-Gal4ff.zf1
(Plasmid #61392)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61392 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4100
  • Total vector size (bp) 4632
  • Vector type
    Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gal4ff.zf1
  • Species
    Synthetic
  • Insert Size (bp)
    540
  • Promoter sp6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-Gal4ff.zf1 was a gift from Harold Burgess (Addgene plasmid # 61392 ; http://n2t.net/addgene:61392 ; RRID:Addgene_61392)

  • For your References section:

    Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish. Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, Burgess HA. Nucleic Acids Res. 2015 Jan 27. pii: gkv035. 10.1093/nar/gkv035 PubMed 25628360
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