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Addgene

pEGFP-N1-hRIP1-FL I541P
(Plasmid #61381)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61381 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Full length human RIP1with I541P mutation
  • Species
    H. sapiens (human)
  • Mutation
    changed I541 to P
  • Entrez Gene
    RIPK1 (a.k.a. AIEFL, IMD57, RIP, RIP-1, RIP1)
  • Tags / Fusion Proteins
    • HA tag (N terminal on insert)
    • EGFP (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The hRIP1 insert contains a E96G substitution that does not affect function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1-hRIP1-FL I541P was a gift from Francis Chan (Addgene plasmid # 61381 ; http://n2t.net/addgene:61381 ; RRID:Addgene_61381)

  • For your References section:

    The RIP1/RIP3 necrosome forms a functional amyloid signaling complex required for programmed necrosis. Li J, McQuade T, Siemer AB, Napetschnig J, Moriwaki K, Hsiao YS, Damko E, Moquin D, Walz T, McDermott A, Chan FK, Wu H. Cell. 2012 Jul 20;150(2):339-50. doi: 10.1016/j.cell.2012.06.019. 10.1016/j.cell.2012.06.019 PubMed 22817896
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