pEGFP-N1-hRIP1-FL I539P
(Plasmid
#61380)
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PurposeExpresses Human full length RIPK1with I539P mutation in the mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61380 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFull length human RIP1with I539P mutation
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SpeciesH. sapiens (human)
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Mutationchanged I539 to P
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Entrez GeneRIPK1 (a.k.a. AIEFL, IMD57, RIP, RIP-1, RIP1)
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Tags
/ Fusion Proteins
- HA tag (N terminal on insert)
- EGFP (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The hRIP1 insert contains a E96G substitution that does not affect function
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-N1-hRIP1-FL I539P was a gift from Francis Chan (Addgene plasmid # 61380 ; http://n2t.net/addgene:61380 ; RRID:Addgene_61380) -
For your References section:
The RIP1/RIP3 necrosome forms a functional amyloid signaling complex required for programmed necrosis. Li J, McQuade T, Siemer AB, Napetschnig J, Moriwaki K, Hsiao YS, Damko E, Moquin D, Walz T, McDermott A, Chan FK, Wu H. Cell. 2012 Jul 20;150(2):339-50. doi: 10.1016/j.cell.2012.06.019. 10.1016/j.cell.2012.06.019 PubMed 22817896