pME-QS (L1-L2)
(Plasmid
#61378)
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PurposeGateway Middle Entry Vector Containing QS
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61378 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 2555
- Total vector size (bp) 6207
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameQS
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Insert Size (bp)3652
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 17mer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Discrepancies between full sequence and Addgene's QC results have no functional consequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-QS (L1-L2) was a gift from Marnie Halpern (Addgene plasmid # 61378 ; http://n2t.net/addgene:61378 ; RRID:Addgene_61378) -
For your References section:
Adoption of the Q transcriptional regulatory system for zebrafish transgenesis. Subedi A, Macurak M, Gee ST, Monge E, Goll MG, Potter CJ, Parsons MJ, Halpern ME. Methods. 2014 Apr 1;66(3):433-40. doi: 10.1016/j.ymeth.2013.06.012. Epub 2013 Jun 20. 10.1016/j.ymeth.2013.06.012 PubMed 23792917