pME-QF
(Plasmid
#61375)
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PurposeMiddle Gateway Entry Vector Containing QF
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61375 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 2564
- Total vector size (bp) 5724
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameQF
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Alt nameQuinate activator
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SpeciesNeurospora
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Insert Size (bp)3160
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer m13 17mer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-QF was a gift from Marnie Halpern (Addgene plasmid # 61375 ; http://n2t.net/addgene:61375 ; RRID:Addgene_61375) -
For your References section:
Adoption of the Q transcriptional regulatory system for zebrafish transgenesis. Subedi A, Macurak M, Gee ST, Monge E, Goll MG, Potter CJ, Parsons MJ, Halpern ME. Methods. 2014 Apr 1;66(3):433-40. doi: 10.1016/j.ymeth.2013.06.012. Epub 2013 Jun 20. 10.1016/j.ymeth.2013.06.012 PubMed 23792917