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Purpose5' Gateway Entry Clone Containing 4xnrUAS
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61372 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONOR P4-P1R
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Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 2645
- Total vector size (bp) 2820
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert name4xnrUAS
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Insert Size (bp)175
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer m13 17mer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5E-4xnrUAS (L4-R1) was a gift from Marnie Halpern (Addgene plasmid # 61372 ; http://n2t.net/addgene:61372 ; RRID:Addgene_61372) -
For your References section:
Transgenerational analysis of transcriptional silencing in zebrafish. Akitake CM, Macurak M, Halpern ME, Goll MG. Dev Biol. 2011 Apr 15;352(2):191-201. doi: 10.1016/j.ydbio.2011.01.002. Epub 2011 Jan 9. 10.1016/j.ydbio.2011.01.002 PubMed 21223961