pcDNA3.3-Nm-dCas9-p300 Core
(Plasmid #61365)

• Purpose: encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
• Depositing Lab: Charles Gersbach
• Publication: Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.

Backbone

• Vector backbone: pcDNA3.3
• Backbone manufacturer: Invitrogen; Life Technologies
• Total vector size (bp): 10579
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Nm-dCas9-p300 Core
• Species: H. sapiens (human), Synthetic; Neisseria meningiditis
• Insert Size (bp): 5175
• Mutation: D16A, D587A, H588A, N611A in Nm Cas9
• GenBank ID: WP_002235162.1 NP_001420.2
• Entrez Gene: EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)
• Promoter: CMV
• Tags / Fusion Proteins:
  • NLS (C terminal on insert)
  • HA tag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV F
• 3′ sequencing primer: TK-pA R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry