pcDNA-dCas9-p300 Core (1645/1646 RR/EE)
(Plasmid #61359)

• Purpose: encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
• Depositing Lab: Charles Gersbach
• Publication: Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen; Life Technologies
• Total vector size (bp): 11475
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300)
• Species: H. sapiens (human), Synthetic; S. pyogenes
• Insert Size (bp): 6138
• Mutation: D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE mutation in p300 Core
• GenBank ID: WP_010922251.1 NP_001420.2
• Entrez Gene: EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • SV40 NLS (N terminal on insert)
  • SV40 NLS (C terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: CMV F
• 3′ sequencing primer: Bgh R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry