pcDNA-dCas9-p300 Core Citations (46)
Originally described in: Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy TE, Gersbach CA Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. PubMed Journal
Articles Citing pcDNA-dCas9-p300 Core
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| CRISPR-mediated Activation of Latent HIV-1 Expression. Limsirichai P, Gaj T, Schaffer DV. Mol Ther. 2015 Nov 26. doi: 10.1038/mt.2015.213. PubMed |
| Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Jusiak B, Cleto S, Perez-Pinera P, Lu TK. Trends Biotechnol. 2016 Jan 22. pii: S0167-7799(15)00274-7. doi: 10.1016/j.tibtech.2015.12.014. PubMed |
Comparison of Cas9 activators in multiple species.
Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church G.
Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
PubMed
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| Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system. Garcia-Bloj B, Moses C, Sgro A, Plani-Lam J, Arooj M, Duffy C, Thiruvengadam S, Sorolla A, Rashwan R, Mancera RL, Leisewitz A, Swift-Scanlan T, Corvalan AH, Blancafort P. Oncotarget. 2016 Sep 13;7(37):60535-60554. doi: 10.18632/oncotarget.11142. PubMed |
| Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing. Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A. Cell. 2016 Sep 22;167(1):219-232.e14. doi: 10.1016/j.cell.2016.09.006. PubMed |
| Targeted Gene Activation Using RNA-Guided Nucleases. Brown A, Woods WS, Perez-Pinera P. Methods Mol Biol. 2017;1468:235-50. doi: 10.1007/978-1-4939-4035-6_16. PubMed |
CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome.
Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA.
Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
PubMed
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| Ethanol Exposure Regulates Gabra1 Expression via Histone Deacetylation at the Promoter in Cultured Cortical Neurons. Bohnsack JP, Patel VK, Morrow AL. J Pharmacol Exp Ther. 2017 Oct;363(1):1-11. doi: 10.1124/jpet.117.242446. Epub 2017 Aug 10. PubMed |
| CRISPR-Based Chromatin Remodeling of the Endogenous Oct4 or Sox2 Locus Enables Reprogramming to Pluripotency. Liu P, Chen M, Liu Y, Qi LS, Ding S. Cell Stem Cell. 2018 Feb 1;22(2):252-261.e4. doi: 10.1016/j.stem.2017.12.001. Epub 2018 Jan 18. PubMed |
| Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells. Gao D, Liang FS. Methods Mol Biol. 2018;1767:429-445. doi: 10.1007/978-1-4939-7774-1_24. PubMed |
| A novel enhancer regulates MGMT expression and promotes temozolomide resistance in glioblastoma. Chen X, Zhang M, Gan H, Wang H, Lee JH, Fang D, Kitange GJ, He L, Hu Z, Parney IF, Meyer FB, Giannini C, Sarkaria JN, Zhang Z. Nat Commun. 2018 Jul 27;9(1):2949. doi: 10.1038/s41467-018-05373-4. PubMed |
| Temporal and Spatial Epigenome Editing Allows Precise Gene Regulation in Mammalian Cells. Kuscu C, Mammadov R, Czikora A, Unlu H, Tufan T, Fischer NL, Arslan S, Bekiranov S, Kanemaki M, Adli M. J Mol Biol. 2019 Jan 4;431(1):111-121. doi: 10.1016/j.jmb.2018.08.001. Epub 2018 Aug 9. PubMed |
| Super-enhancers maintain renin-expressing cell identity and memory to preserve multi-system homeostasis. Martinez MF, Medrano S, Brown EA, Tufan T, Shang S, Bertoncello N, Guessoum O, Adli M, Belyea BC, Sequeira-Lopez MLS, Gomez RA. J Clin Invest. 2018 Nov 1;128(11):4787-4803. doi: 10.1172/JCI121361. Epub 2018 Oct 2. PubMed |
| Rationally-engineered reproductive barriers using CRISPR & CRISPRa: an evaluation of the synthetic species concept in Drosophila melanogaster. Waters AJ, Capriotti P, Gaboriau DCA, Papathanos PA, Windbichler N. Sci Rep. 2018 Sep 3;8(1):13125. doi: 10.1038/s41598-018-31433-2. PubMed |
Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing.
Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB.
Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286.
PubMed
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| CRISPR-based Activation of Endogenous Neurotrophic Genes in Adipose Stem Cell Sheets to Stimulate Peripheral Nerve Regeneration. Hsu MN, Liao HT, Truong VA, Huang KL, Yu FJ, Chen HH, Nguyen TKN, Makarevich P, Parfyonova Y, Hu YC. Theranostics. 2019 Aug 14;9(21):6099-6111. doi: 10.7150/thno.36790. eCollection 2019. PubMed |
| Sufficiency analysis of estrogen responsive enhancers using synthetic activators. Ginley-Hidinger M, Carleton JB, Rodriguez AC, Berrett KC, Gertz J. Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900497. doi: 10.26508/lsa.201900497. Print 2019 Oct. PubMed |
| ATM and ATR Expression Potentiates HBV Replication and Contributes to Reactivation of HBV Infection upon DNA Damage. Kostyusheva A, Brezgin S, Bayurova E, Gordeychuk I, Isaguliants M, Goptar I, Urusov F, Nikiforova A, Volchkova E, Kostyushev D, Chulanov V. Viruses. 2019 Oct 31;11(11). pii: v11110997. doi: 10.3390/v11110997. PubMed |
| Epigenetic therapy of novel tumour suppressor ZAR1 and its cancer biomarker function. Deutschmeyer V, Breuer J, Walesch SK, Sokol AM, Graumann J, Bartkuhn M, Boettger T, Rossbach O, Richter AM. Clin Epigenetics. 2019 Dec 4;11(1):182. doi: 10.1186/s13148-019-0774-2. PubMed |
Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing.
Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson KE, Ni M, Xu J.
Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5.
PubMed
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Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9.
Liu X, Chen Y, Zhang Y, Liu Y, Liu N, Botten GA, Cao H, Orkin SH, Zhang MQ, Xu J.
Genome Biol. 2020 Mar 5;21(1):59. doi: 10.1186/s13059-020-01973-w.
PubMed
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| Noncoding Variants Connect Enhancer Dysregulation with Nuclear Receptor Signaling in Hematopoietic Malignancies. Li K, Zhang Y, Liu X, Liu Y, Gu Z, Cao H, Dickerson KE, Chen M, Chen W, Shao Z, Ni M, Xu J. Cancer Discov. 2020 May;10(5):724-745. doi: 10.1158/2159-8290.CD-19-1128. Epub 2020 Mar 18. PubMed |
| Reversible promoter methylation determines fluctuating expression of acute phase proteins. Zhang SC, Wang MY, Feng JR, Chang Y, Ji SR, Wu Y. Elife. 2020 Mar 30;9. pii: 51317. doi: 10.7554/eLife.51317. PubMed |
| KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain. Lipinski M, Munoz-Viana R, Del Blanco B, Marquez-Galera A, Medrano-Relinque J, Carames JM, Szczepankiewicz AA, Fernandez-Albert J, Navarron CM, Olivares R, Wilczynski GM, Canals S, Lopez-Atalaya JP, Barco A. Nat Commun. 2020 May 22;11(1):2588. doi: 10.1038/s41467-020-16246-0. PubMed |
Enhancer RNAs predict enhancer-gene regulatory links and are critical for enhancer function in neuronal systems.
Carullo NVN, Phillips Iii RA, Simon RC, Soto SAR, Hinds JE, Salisbury AJ, Revanna JS, Bunner KD, Ianov L, Sultan FA, Savell KE, Gersbach CA, Day JJ.
Nucleic Acids Res. 2020 Aug 18. pii: 5893972. doi: 10.1093/nar/gkaa671.
PubMed
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| Clearing of Foreign Episomal DNA from Human Cells by CRISPRa-Mediated Activation of Cytidine Deaminases. Brezgin S, Kostyusheva A, Ponomareva N, Volia V, Goptar I, Nikiforova A, Shilovskiy I, Smirnov V, Kostyushev D, Chulanov V. Int J Mol Sci. 2020 Sep 18;21(18). pii: ijms21186865. doi: 10.3390/ijms21186865. PubMed |
| BAHCC1 binds H3K27me3 via a conserved BAH module to mediate gene silencing and oncogenesis. Fan H, Lu J, Guo Y, Li D, Zhang ZM, Tsai YH, Pi WC, Ahn JH, Gong W, Xiang Y, Allison DF, Geng H, He S, Diao Y, Chen WY, Strahl BD, Cai L, Song J, Wang GG. Nat Genet. 2020 Dec;52(12):1384-1396. doi: 10.1038/s41588-020-00729-3. Epub 2020 Nov 2. PubMed |
| Profiling of H3K27Ac Reveals the Influence of Asthma on the Epigenome of the Airway Epithelium. McErlean P, Kelly A, Dhariwal J, Kirtland M, Watson J, Ranz I, Smith J, Saxena A, Cousins DJ, Van Oosterhout A, Solari R, Edwards MR, Johnston SL, Lavender P. Front Genet. 2020 Dec 10;11:585746. doi: 10.3389/fgene.2020.585746. eCollection 2020. PubMed |
| Regulation of MYB by distal enhancer elements in human myeloid leukemia. Li M, Jiang P, Cheng K, Zhang Z, Lan S, Li X, Zhao L, Wang Y, Wang X, Chen J, Ji T, Han B, Zhang J. Cell Death Dis. 2021 Feb 26;12(2):223. doi: 10.1038/s41419-021-03515-z. PubMed |
| Lupus susceptibility region containing CDKN1B rs34330 mechanistically influences expression and function of multiple target genes, also linked to proliferation and apoptosis. Singh B, Maiti GP, Zhou X, Fazel-Najafabadi M, Bae SC, Sun C, Terao C, Okada Y, Chua KH, Kochi Y, Guthridge JM, Zhang H, Weirauch M, James JA, Harley JB, Varshney GK, Looger LL, Nath SK. Arthritis Rheumatol. 2021 May 13. doi: 10.1002/art.41799. PubMed |
| VEGFA's distal enhancer regulates its alternative splicing in CML. Dahan S, Sharma A, Cohen K, Baker M, Taqatqa N, Bentata M, Engal E, Siam A, Kay G, Drier Y, Elias S, Salton M. NAR Cancer. 2021 Jul 13;3(3):zcab029. doi: 10.1093/narcan/zcab029. eCollection 2021 Sep. PubMed |
| Bi-directional gene activation and repression promote ASC differentiation and enhance bone healing in osteoporotic rats. Truong VA, Lin YH, Nguyen NTK, Hsu MN, Pham NN, Chang YH, Chang CW, Shen CC, Lee HS, Lai PL, Parfyonova YV, Menshikov M, Wu JC, Chang YH, Hu YC. Mol Ther. 2022 Jan 5;30(1):92-104. doi: 10.1016/j.ymthe.2021.08.024. Epub 2021 Aug 25. PubMed |
Modular Synthetic Biology Toolkit for Filamentous Fungi.
Mozsik L, Pohl C, Meyer V, Bovenberg RAL, Nygard Y, Driessen AJM.
ACS Synth Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260.
PubMed
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| Histone marks regulate the epithelial-to-mesenchymal transition via alternative splicing. Segelle A, Nunez-Alvarez Y, Oldfield AJ, Webb KM, Voigt P, Luco RF. Cell Rep. 2022 Feb 15;38(7):110357. doi: 10.1016/j.celrep.2022.110357. PubMed |
| CRISPR-Mediated Synergistic Epigenetic and Transcriptional Control. Dominguez AA, Chavez MG, Urke A, Gao Y, Wang L, Qi LS. CRISPR J. 2022 Apr;5(2):264-275. doi: 10.1089/crispr.2021.0099. Epub 2022 Mar 10. PubMed |
| Shear stress switches the association of endothelial enhancers from ETV/ETS to KLF transcription factor binding sites. Tsaryk R, Yucel N, Leonard EV, Diaz N, Bondareva O, Odenthal-Schnittler M, Arany Z, Vaquerizas JM, Schnittler H, Siekmann AF. Sci Rep. 2022 Mar 21;12(1):4795. doi: 10.1038/s41598-022-08645-8. PubMed |
Aberrant promoter methylation contributes to LRIG1 silencing in basal/triple-negative breast cancer.
Umeh-Garcia M, O'Geen H, Simion C, Gephart MH, Segal DJ, Sweeney CA.
Br J Cancer. 2022 Apr 19. pii: 10.1038/s41416-022-01812-8. doi: 10.1038/s41416-022-01812-8.
PubMed
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| The Activation of Protamine 1 Using Epigenome Editing Decreases the Proliferation of Tumorigenic Cells. Namous H, Braz CU, Wang Y, Khatib H. Front Genome Ed. 2022 Jun 16;4:844904. doi: 10.3389/fgeed.2022.844904. eCollection 2022. PubMed |
| Epigenetically silenced apoptosis-associated tyrosine kinase (AATK) facilitates a decreased expression of Cyclin D1 and WEE1, phosphorylates TP53 and reduces cell proliferation in a kinase-dependent manner. Woods ML, Weiss A, Sokol AM, Graumann J, Boettger T, Richter AM, Schermuly RT, Dammann RH. Cancer Gene Ther. 2022 Dec;29(12):1975-1987. doi: 10.1038/s41417-022-00513-x. Epub 2022 Jul 28. PubMed |
| XLID syndrome gene Med12 promotes Ig isotype switching through chromatin modification and enhancer RNA regulation. Haque F, Honjo T, Begum NA. Sci Adv. 2022 Nov 25;8(47):eadd1466. doi: 10.1126/sciadv.add1466. Epub 2022 Nov 25. PubMed |
| Targeted Modulation of Chicken Genes In Vitro Using CRISPRa and CRISPRi Toolkit. Chapman B, Han JH, Lee HJ, Ruud I, Kim TH. Genes (Basel). 2023 Apr 13;14(4):906. doi: 10.3390/genes14040906. PubMed |
| Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus. Kostyushev D, Brezgin S, Kostyusheva A, Ponomareva N, Bayurova E, Zakirova N, Kondrashova A, Goptar I, Nikiforova A, Sudina A, Babin Y, Gordeychuk I, Lukashev A, Zamyatnin AA Jr, Ivanov A, Chulanov V. Mol Ther Nucleic Acids. 2023 Apr 20;32:478-493. doi: 10.1016/j.omtn.2023.04.016. eCollection 2023 Jun 13. PubMed |
Isoforms of the TAL1 transcription factor have different roles in hematopoiesis and cell growth.
Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, Plaschkes I, Kay G, Drier Y, Berger M, Salton M.
PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 10.1371/journal.pbio.3002175.
PubMed
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| HDAC10 blockade upregulates SPARC expression thereby repressing melanoma cell growth and BRAF inhibitor resistance. Ling H, Li Y, Peng C, Yang S, Seto E. bioRxiv [Preprint]. 2023 Dec 7:2023.12.05.570182. doi: 10.1101/2023.12.05.570182. PubMed |
| ITGB2 is a central hub-gene associated with inflammation and early fibro-atheroma development in a swine model of atherosclerosis. Namous H, Strillacci MG, Braz CU, Shanmuganayagam D, Krueger C, Peppas A, Soffregen WC, Reed J, Granada JF, Khatib H. Atheroscler Plus. 2023 Nov 15;54:30-41. doi: 10.1016/j.athplu.2023.11.001. eCollection 2023 Dec. PubMed |
| HDAC10 inhibition represses melanoma cell growth and BRAF inhibitor resistance via upregulating SPARC expression. Ling H, Li Y, Peng C, Yang S, Seto E. NAR Cancer. 2024 Apr 22;6(2):zcae018. doi: 10.1093/narcan/zcae018. eCollection 2024 Jun. PubMed |
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